Caspian Journal of Pediatrics

en Colonization of rectovaginal Escherichia coli and group B streptococci in mothers and on infants' body surface and their related risk factors عمومى General پژوهشي Research Background: Microorganisms that cause early neonatal sepsis are usually already colonized rectovaginal area in mothers. The most common of these organisms is group B streptococci (GBS) and intestinal gram-negative bacteria mostly Escherichia coli (E.coli). The use of prophylactic antibiotics against GBS has increased in recent years. This study aimed to determine the current situation and frequency of E.coli and GBS colonization in mothers and their infant. Methods: All pregnant women with gestational age≥26 weeks, progressive labor pain and no history of using antibiotic were entered into the current study. A sterile cotton swab culturing from distal third of vaginal and rectum of mothers, and six hours after delivery from external ear canal, nose, groin and umbilicus of infant has been taken. All samples were transferred to the laboratory in Stuart’s media, and then cultured to standard media within 24 hours and the main two organisms in neonatal sepsis (E.coli and GBS) were isolated from mothers' and infants' cultures. Results: E.coli and GBS were 56.3% and 11.2% respectively in rectovaginal culture, and 29.8% and 8.8% in infants’ body surface culture. There was a significant difference in rectovaginal GBS colonization between term (13.6%) and preterm (3.2%) (P=0.005), while the frequency of positive E.coli culture was 52.8% in term deliveries and 68.1% in preterm ones, showing a significant difference (P=0.009). Conclusions: Since E.coli is more common in preterm delivery in this geographical region, in cases of amniotic membrane rupture, mothers should be adequately protected with prophylactic antibiotics against neonatal sepsis. Maternal colonization, Neonatal sepsis, Escherichia coli, Group B Streptococci, Prophylactic antibiotic 148 152 http://caspianjp.ir/browse.php?a_code=A-10-46-1&slc_lang=en&sid=1 Zahra Akbarian Rad zhr_akbarian@yahoo.com 10031947532846001496 10031947532846001496 No Non-Communicable Pediatric Diseases Research Center, Assistant of Neonatology Department, Babol University of Medical Sciences, Babol, Iran Mohsen Haghshenas Mojaveri dr.hagh46@yahoo.com 10031947532846001497 10031947532846001497 No Non-Communicable Pediatric Diseases Research Center, Assistant of Neonatology Department, Babol University of Medical Sciences, Babol, Iran Seddigheh Esmaeilzadeh sesmael@yahoo.com 10031947532846001498 10031947532846001498 Yes Fateme Zahra Fertility & Infertility Research Health Center, Obstetrics & Gynecology Department, Babol of University of Medical Science, Babol, Iran Alireza Firouzjahi firouzal@hotmail.com 10031947532846001499 10031947532846001499 No The Development Clinical Research Development unit of Rouhani Hospital, Department of Pathology, Babol University of Medical Sciences, Iran, Babol Mahsa Laegh mojy_rad@yahoo.com 10031947532846001500 10031947532846001500 No Student Committee Research, Babol University of Medical Sciences, Babol, Iran Soraya Khafri khafri@yahoo.com 10031947532846001501 10031947532846001501 No Department of Statistic and Epidmiology, Babol University of Medical Sciences, Babol, Iran. Mojgan Naeimi Rad mojy_rad@yahoo.com 10031947532846001502 10031947532846001502 No The Development Clinical Research Development unit of Rouhani Hospital, Master of Midwifery, Babol University of Medical Sciences, Iran, Babol